Bioactive molecules modelised by numerical simulation:molecules against Escherichia Coli

Transformation of Escherichia coli with large DNA molecules by electroporation.

We have examined bacterial electroporation with a specific interest in the transformation of large DNA, i.e. molecules > 100 kb. We have used DNA from bacterial artificial chromosomes (BACs) ranging from 7 to 240 kb, as well as BAC ligation mixes containing a range o different sized molecules. The efficiency of electroporation with large DNA is strongly dependent on the strain of Escherichia co...

A DNA Vaccine against Escherichia coli O157:H7

Background: Infection with Escherichia coli O157:H7 rarely leads to bloody diarrhea and causes hemolytic uremic syndrome with renal failure that can be deadly dangerous. Intimin, translocated Intimin receptor (Tir), and enterohemorrhagic E. coli (EHEC) secreted protein A (EspA) proteins are the virulence factors expressed by locus of enterocyte effacement locus of EHEC. This bacterium needs Es...

Chitosan-Based Intranasal Vaccine against Escherichia coli O157:H7

Background: Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an infectious zoonotic pathogen causing human infections. These infections, in some cases, can lead to hemolytic uremic syndrome and its life-threatening complications and even death worldwide. The first intimate bacterial adhesion, intimin (I), with its own receptor translocated intimin receptor (Tir) and E. coli secreted protein...

antibacterial activity of chitosan against escherichia coli

introduction: chitosan possesses more than 5000 glucossamine residue. since chitosan possesses potentially reactive amino functional groups, this polymer has been shown to be of potential use in many different fields, including as an antifungal compound in agriculture, flocculating in waste water treatment, a food additive and alimentary industries, hydrating agent in cosmetics and more recentl...

Case Report of Meningoencephalitis in Calves Caused by Escherichia coli